Physiochemical Properties
Liquid and powder classical media products are tested to determine pH and osmolality. Classical media powders are also tested for residual moisture. The pH and osmolality tests are performed according to specific procedures in which all equipment is calibrated using standards traceable to the National Institute of Standards and Technology.
Biological Tests
Quality release testing of Corning liquid classical media products assures low levels of endotoxin, mycoplasma, and an absence of contamination.
Endotoxin
Classical media products are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) chromogenic assay. For release, each lot of standard liquid classical media must have an endotoxin level of <0.25 EU/mL. Classical media powder products must have an endotoxin level of <0.25 EU/mL when reconstituted to formula weight with low endotoxin water.
Sterility
All Corning liquid classical media products are tested for sterility.
Mycoplasma
Liquid classical media products are tested for mycoplasma using a large-volume method.
Formulation
without Glucose, L-glutamine and Sodium Pyruvate
with 1 g/L Glucose, Sodium Pyruvate and L-glutamine
with 25mM HEPES, 4.5 g/L Glucose, and L-glutamine, without Sodium Pyruvate
with 4.5 g/L Glucose and L-glutamine, without Sodium Pyruvate
with 4.5 g/L Glucose and L-glutamine, without Sodium Pyruvate and Sodium Bicarbonate
with 4.5g/L Glucose, L-glutamine, and Sodium Pyruvate
with 4.5 g/L Glucose, L-glutamine, and Sodium Pyruvate, without Sodium Bicarbonate
[+] 4.5g/L Glucose, L-glutamine, Sodium Pyruvate; [-] Sodium Bicarbonate
with 4.5 g/L Glucose, without L-glutamine and Sodium Pyruvate
with 4.5 g/L Glucose, without L-glutamine, Sodium Pyruvate, and Phenol Red
with 4.5 g/L Glucose, Sodium Pyruvate, and 25 mM HEPES, without L-glutamine
with 4.5g/L Glucose and Sodium Pyruvate, without L-glutamine
with 4.5 g/L Glucose and Sodium Pyruvate, without L-glutamine, L-methionine, and L-cystine
with 4.5 g/L Glucose and Sodium Pyruvate, without L-glutamine and Phenol Red
with 4.5/L Glucose and Sodium Pyruvate, without L-glutamine
[+] Corning glutagro Supplement, 4.5 g/L Glucose, Phenol; [-] Sodium Pyruvate
[+] Corning Glutagro Supplement, 4.5 g/L Glucose, Sodium Pyruvate, Phenol Red
DMEM, [+] 1000 mg/L D-glucose, L-glutamine, 110 mg/L sodium pyruvate, [-] sodium bicarbonate
DMEM, [+] 4500 mg/L D-glucose, L-glutamine, 110 mg/L sodium pyruvate, [-] sodium bicarbonate
DMEM, [+] 4500 mg/L D-glucose, L-glutamine, 25 mM HEPES, [-] sodium pyruvate, sodium bicarbonate
DMEM, [+] 4500 mg/L D-glucose, L-glutamine, [-] phenol red, sodium pyruvate, sodium bicarbonate
DMEM, [+] 4500 mg/L D-glucose, L-glutamine, [-] sodium bicarbonate
Shelf Life
12 Months
18 Months
36 Months
3 Years
Size
100 mL
500 mL
1 L
10 L
20 L
50 L
100 L
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